解剖学报

›› 2010, Vol. 41 ›› Issue (03): 358-361.doi: 10.3969/j.issn.0529-1356.2010.03.006

• 论著 • 上一篇    下一篇

睡眠剥夺后大鼠皮质、海马和杏仁核微管相关蛋白-2及神经丝蛋白的表达变化

王景涛1,2; 邱洪斌1; 田国忠1* ; 扈青云1; 徐辉1; 王柏新1;李廷利2   

  1. 1.佳木斯大学基础医学院，黑龙江 佳木斯 154007; 2.黑龙江省中医药大学科技处，哈尔滨 150040
  • 收稿日期:2009-05-25 修回日期:2009-09-21 出版日期:2010-06-06
  • 通讯作者: 田国忠

Expression of microtubule-associated protein-2 and neurofilament-200 in rat brain after sleep deprivation

  1. 1.Basic Medical College of Jiamusi University, Heilongjiang Jiamusi 154007,China;2.Technology Services, Heilongjiang University of Chinese Medicine, Harbin 150040, China
  • Received:2009-05-25 Revised:2009-09-21 Online:2010-06-06
  • Contact: TIAN Guo-zhong

关键词: 睡眠剥夺, 微管相关蛋白-2, 神经丝蛋白, 免疫组织化学, 大鼠

Abstract: P>Objective To study the expression of microtubule-associated protein-2(MAP-2) and neurofilament-200(NF-200) in cortex, hippocampus and amygdaloid body of rat after sleep deprivation(SD). Methods SD model was made by modified multiple platform method（MMPM）, rats were divided into sleep deprivation group(SD),tank control group(TC), cage control group(CC). The sleep deprivation group included seven time-length groups (sleep deprivation 6 hours,12 hours,1 day,2 days,3 days,5 days and 7 days). Five rats were assigned to each group. The expression of MAP-2 and NF-200 were detected by immunohistochemical method. Results At the 5th day and 7th day, we found that the density of MAP-2 and NF-200 positive neuron decreased in cortex, CA1,CA2,CA3,dentate gyrus and amygdaloid body（P0.05，P0.01). Conclusio

Key words: Sleep deprivation(SD), MAP-2, NF-200, Immuno histochemistry, Rat

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